Peggy Sue reagents and consumables
Peggy Sue reagents and consumables are designed for use on with Peggy Sue and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.
Size Assay Modules
| Part # | Description |
|---|---|
| DM-001 | Anti-Rabbit Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP. |
| DM-002 | Anti-Mouse Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP. |
| DM-003 | No secondary Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP. |
| DM-004 | Biotin Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder). Compatible with Separation module - sold separately. |
| DM-005 | Anti-Human IgG Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-006 | Anti-Goat Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-TP01 | Total Protein Detection Module for Chemiluminescence Based Total Protein Assays Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The Total protein detection module for Chemiluminescence based total protein assays includes Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP. Compatible with Separation module - sold separately. |
| SM-S001 | 12-230 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 2, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C). |
| SM-S002 | 66-440 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 3, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 3, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C). |
| SM-S003 | 2-40 kDa Peggy Sue or Sally Sue Separation Module Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 4, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C). |
Charge Assay Kits
| Part # | Description |
|---|---|
| CBS2001 | XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges. |
| CBS404 | HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS403 | Lysis Kit - Bicine/CHAPS Buffer for Charge Assays Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS401 | Lysis Kit - RIPA Buffer for Charge Assays Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| 041-126 | Amplified Rabbit Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-127 | Amplified Mouse Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| PS-T003 | Peggy System Charge Training Kit Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents |
Individual Reagents
| Part # | Description |
|---|---|
| PS-ST05EZ-8 | EZ Standard Pack 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa) and Single Use FL Standard (26 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST04EZ-8 | EZ Standard Pack 4 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (200 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST03EZ-8 | EZ Standard Pack 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (90 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST02EZ-8 | EZ Standard Pack 2 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (180 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST01EZ-8 | EZ Standard Pack 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (29 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-FL01-8 | Fluorescent 5x Master Mix 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Compatible with 12-230 Separation Modules - sold separately. |
| PS-FL03-8 | Fluorescent 5x Master Mix 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. Compatible with 66-440 kD Separation Modules - sold separately. |
| PS-FL05-8 | Fluorescent 5x Master Mix 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. Compatible with 2-40 kD Separation Modules - sold separately. |
| 043-226 | Peggy Sue or Sally Sue 66-440 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-209) and Lower (043-210) Running buffers 3. |
| 043-225 | Peggy Sue or Sally Sue 12-230 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-163) and Lower (043-164) Running buffers 2. |
| 043-326 | Peggy Sue or Sally Sue 2-40 kDa Split Running Buffer 4 Kit Requires Compass v3.0 or higher. Includes both the Upper (043-329) and Lower (043-330) Running buffers 4 |
| 042-976 | Total Protein Streptavidin-HRP Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems., 2.0 mL. Ready to use. |
| 042-918 | Peggy Sue/Sally Sue 66-440 kDa Separation Matrix Use with Compass v2.6 or higher. Separation Matrix 3 for Peggy Sue or Sally Sue (2.5 mL) |
| 043-328 | Peggy Sue/Sally Sue 2-40 kDa Separation Matrix Requires Compass v3.0 or higher. Separation Matrix 4 for Peggy Sue or Sally Sue (2.5 mL) |
| 042-512 | Separation Matrix II (2.5 mL) for Peggy Sue or Sally Sue Separation matrix II (2.5 mL) |
| 042-513 | Stacking Matrix for Peggy Sue or Sally Sue Stacking matrix II (1.5 mL) |
| 042-195 | Sample Buffer Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-206 | Anti-Rabbit Secondary Antibody Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-205 | Anti-Mouse Secondary Antibody Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-426 | 20X Anti-Rabbit HRP Conjugate 20X Anti-Rabbit HRP Conjugate, 150 µL for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit. |
| 042-203 | Antibody Diluent 2 Antibody Diluent 2 (20 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. |
| 042-196 | 10x System Control Primary Antibody (Rabbit) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial). |
| 042-191 | 10x System Control Primary Antibody (Mouse) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The 10X System Control Primary Antibody-Mouse detects system controls is to be used with Antibody Diluent. *Please match accordingly with Standard packs 1, 2, 3 or 4 (250 µl/vial). |
| 042-518 | Matrix Removal Buffer for Peggy Sue or Sally Sue Matrix Removal Buffer II (140 mL) |
| 042-414 | Streptavidin-HRP Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard. |
| 042-520 | Wash Buffer for Peggy Sue or Sally Sue Wash Buffer (170 mL) |
| 043-311 | Luminol-S Luminol-S (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-379 | Peroxide Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-486 | ERK 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-488 | HeLa Lysate Controls HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-522-2 | Anti-Goat Secondary HRP Antibody Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. ProteinSimple recommends the use of our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. |
| 043-491-2 | Anti-Human IgG Secondary HRP Antibody Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-459-2 | Secondary Streptavidin-HRP Secondary Streptavidin-HRP Conjugate (ready-to-use reagent) for use with a biotinylated antibody in Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. For total protein assay use Secondary Streptavidin-HRP (P/N: 042-976) and to detect biotinylated molecular weight ladders use Streptavidin-HRP (P/N: 042-414). |
| 043-524 | Milk-free Antibody Diluent Milk-free Antibody Diluent compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. |
| 040-655 | Goat-Anti-Mouse-HRP Secondary Antibody HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-656 | Goat-Anti-Rabbit Secondary Antibody HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-081 | Goat-Anti-Rabbit HUX HRP Secondary Antibody HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-474 | Pan Erk Primary Antibody Pan Erk Primary Antibody, 1X stock (1 mL) |
| 040-477 | Phospho-Erk 1/2 Primary Antibody Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-644 | pI Standard Ladder 1 pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-645 | pI Standard Ladder 2 pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-646 | pI Standard Ladder 3 pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-647 | pI Standard Ladder 4 pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-024 | pI Standard, 4.0 pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-025 | pI Standard, 4.2 pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-026 | pI Standard, 4.4 pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-027 | pI Standard, 4.9 pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-028 | pI Standard, 5.5 pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-029 | pI Standard, 6.0 pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-030 | pI Standard, 6.4 pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-031 | pI Standard, 7.0 pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-032 | pI Standard, 7.3 pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-036 | pI Standard, 8.4 pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-790 | pI Standard, 9.7 pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-967 | Premix G2, ampholyte-free separation gradient Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-968 | Premix G2, pH 3–10 separation gradient Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-970 | Premix G2, pH 4–7 (nested) separation gradient Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-969 | Premix G2, pH 4–9 separation gradient Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-972 | Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-973 | Premix G2, Pharmalyte pH 5–8 separation gradient Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-974 | Premix G2, Servalyt pH 5–8 separation gradient Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-337 | Anolyte Refill Anolyte Refill (200 mL) |
| 040-338 | Catholyte Refill Catholyte Refill (200 mL) |
| 041-108 | Wash Concentrate Refill for Peggy or NanoPro 1000 Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-652 | Luminol Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-483 | RIPA Lysis Buffer RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-764 | Bicine/CHAPS Lysis Buffer and Sample Diluent Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-510 | DMSO Inhibitor Mix DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-482 | Aqueous Inhibitor Mix Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C. |
| 040-649 | Sample Diluent Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-309 | Antibody Diluent Antibody Diluent (10 mL) |
Consumables
| Part # | Description |
|---|---|
| SS701 | Capillaries - Size Separation for Peggy Sue/Sally Sue (5 pack) Peggy/Sally and Peggy Sue/Sally Sue Capillaries for size assays (5 boxes of 96 capillaries) |
| SS700 | Capillaries - Size Separation for Peggy Sue/Sally Sue (1 pack) Peggy Sue/Sally Sue Capillaries for size assays (1 box of 96 capillaries) |
| 031-102 | Repeater Pipette for Peggy Sue or Sally Sue Rainin AR-E1 positive-displacement electronic repeater pipette (0.1 - 50 mL). Use to dispense the Separation Matrix II for Sally Sue and Peggy Sue. |
| 031-103 | Repeater Pipette 0.5 mL Tips for Peggy Sue or Sally Sue A box of 100 of the 0.5 mL syringe tips used with the Peggy Sue/Sally Sue Repeater Pipette. |
| 040-663 | Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes five 384-well plates and lids. * Must be ordered with p/n 041-528. |
| 041-528 | Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663. |
| 041-606 | Pipetting Guide Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay |
| 041-595 | Instrument Cup (set of 3) Includes set of 3 instrument cups |