Wes reagents and consumables
Wes reagents and consumables are designed for use with Wes and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.
Assay Modules
| Part # | Description |
|---|---|
| DM-001 | Anti-Rabbit Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP. |
| DM-002 | Anti-Mouse Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP. |
| DM-003 | No secondary Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP. |
| DM-004 | Biotin Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder). Compatible with Separation module - sold separately. |
| DM-005 | Anti-Human IgG Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-006 | Anti-Goat Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-TP01 | Total Protein Detection Module for Chemiluminescence Based Total Protein Assays Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The Total protein detection module for Chemiluminescence based total protein assays includes Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP. Compatible with Separation module - sold separately. |
| SM-W001 | 12-230 kDa Separation Module, 2 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x13 capillaries per cartridge (26 data points). |
| SM-W002 | 12-230 kDa Separation Module, 8 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 8x13 capillaries per cartridge (104 data points). |
| SM-W003 | 12-230 kDa Separation Module, 2 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x25 capillaries per cartridge (50 data points). |
| SM-W004 | 12-230 kDa Separation Module, 8 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately.Sizing: 8x25 capillaries per cartridge (200 data points). |
| SM-W005 | 66-440 kDa Separation Module, 2 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x13 capillaries per cartridge (26 data points). |
| SM-W006 | 66-440 kDa Separation Module, 8 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 8x13 capillaries per cartridge (104 data points). |
| SM-W007 | 66-440 kDa Separation Module, 2 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x25 capillaries per cartridge (50 data points). |
| SM-W008 | 66-440 kDa Separation Module, 8 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems. Includes separation box with Capillary Cartridges, 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 8x25 capillaries per cartridge (200 data points). |
| SM-W009 | 2-40 kDa Separation Module, 2 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems using Compass v3.0 or higher. Includes separation box with Capillary Cartridges, 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x13 capillaries per cartridge (26 data points). |
| SM-W010 | 2-40 kDa Separation Module, 8 x 13 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems using Compass v3.0 or higher. Includes separation box with Capillary Cartridges, 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 8x13 capillaries per cartridge (104 data points). |
| SM-W011 | 2-40 kDa Separation Module, 2 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems using Compass v3.0 or higher. Includes separation box with Capillary Cartridges, 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 2x25 capillaries per cartridge (50 data points). |
| SM-W012 | 2-40 kDa Separation Module, 8 x 25 capillary cartridges Compatible with Jess™, Abby™ and Wes™ systems using Compass v3.0 or higher. Includes separation box with Capillary Cartridges, 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer, plus Standard Pack 1 clamshell with DTT, FL Standard master mix with 29 kDa System Control protein. Compatible with Detection module - sold separately. Sizing: 8x25 capillaries per cartridge (200 data points). |
| PS-T001 | Chemiluminescence Training Kit Compatible with Jess™, Abby™, and Wes™ systems. Includes a 12-230 kDa Separation Module (SM-W004), Anti-Rabbit Detection Module (DM-001), 10X System Control Primary Antibody, ERK1 Primary Antibody, Anti-Mouse Secondary Antibody, and HeLa Lysate Control for Size. |
| SA-001 | SARS-CoV2 Multi-Antigen Serology Module Compatible with Jess™, Abby™ and Wes™ systems. The SARS-CoV-2 Multi-Antigen Serology Module provides all the reagents needed to detect SARS-CoV-2 IgG antibodies in human serum or plasma in a Simple Western size-based assay when paired with a Separation Module. Please note that this module is for research use only. |
Individual Reagents
| Part # | Description |
|---|---|
| PS-ST05EZ-8 | EZ Standard Pack 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa) and Single Use FL Standard (26 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST04EZ-8 | EZ Standard Pack 4 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (200 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST03EZ-8 | EZ Standard Pack 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (90 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST02EZ-8 | EZ Standard Pack 2 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (180 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST01EZ-8 | EZ Standard Pack 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (29 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-FL01-8 | Fluorescent 5x Master Mix 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Compatible with 12-230 Separation Modules - sold separately. |
| PS-FL03-8 | Fluorescent 5x Master Mix 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. Compatible with 66-440 kD Separation Modules - sold separately. |
| PS-FL05-8 | Fluorescent 5x Master Mix 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. Compatible with 2-40 kD Separation Modules - sold separately. |
| 042-976 | Total Protein Streptavidin-HRP Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems., 2.0 mL. Ready to use. |
| 042-414 | Streptavidin-HRP Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard. |
| 042-195 | Sample Buffer Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-206 | Anti-Rabbit Secondary Antibody Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-205 | Anti-Mouse Secondary Antibody Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-426 | 20X Anti-Rabbit HRP Conjugate 20X Anti-Rabbit HRP Conjugate, 150 µL for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit. |
| 042-203 | Antibody Diluent 2 Antibody Diluent 2 (20 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. |
| 042-196 | 10x System Control Primary Antibody (Rabbit) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial). |
| 042-191 | 10x System Control Primary Antibody (Mouse) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The 10X System Control Primary Antibody-Mouse detects system controls is to be used with Antibody Diluent. *Please match accordingly with Standard packs 1, 2, 3 or 4 (250 µl/vial). |
| 042-202 | Wash Buffer Wash Buffer (60 mL) for use on Jess™, Abby™ and Wes™ systems. |
| PS-CS01 | Chemiluminescent Substrate Compatible with Jess™, Abby™ and Wes™ systems. The chemiluminescent Substrate includes Luminol-S (P/N: 042-233) and Peroxide (P/N: 042-234). Each order contains two bottles of 2.0mL of substrate/bottle. |
| 042-486 | ERK 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-488 | HeLa Lysate Controls HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-311 | Luminol-S Luminol-S (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-379 | Peroxide Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-522-2 | Anti-Goat Secondary HRP Antibody Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. ProteinSimple recommends the use of our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. |
| 043-491-2 | Anti-Human IgG Secondary HRP Antibody Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-459-2 | Secondary Streptavidin-HRP Secondary Streptavidin-HRP Conjugate (ready-to-use reagent) for use with a biotinylated antibody in Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. For total protein assay use Secondary Streptavidin-HRP (P/N: 042-976) and to detect biotinylated molecular weight ladders use Streptavidin-HRP (P/N: 042-414). |
| 043-524 | Milk-free Antibody Diluent Milk-free Antibody Diluent compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. |
Consumables
| Part # | Description |
|---|---|
| PS-PP05 | 2-40 kDa Pre-filled Plates Compatible with Jess™, Abby™ and Wes™ systems using Compass v3.0 or higher. Includes 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer and Matrix Removal Buffer). |
| PS-PP04 | 66-440 kDa Pre-filled Plates Compatible with Jess™, Abby™ and Wes™ systems using Compass v2.7 or higher. Includes 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 3 and Matrix Removal Buffer). |
| PS-PP03 | 12-230 kDa Pre-filled Plates Compatible with Jess™, Abby™ and Wes™ systems using Compass v2.7 or higher. Includes 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 2 and Matrix Removal Buffer). |
| PS-CC01 | 25-Capillary cartridges for Size based Separation Compatible with Jess™, Abby™ and Wes™ systems ONLY. Includes 8 pack of capillaries required to perform for size assays. Sizing: 8 cartridges - 25 capillaries/cartridge. |
| PS-CC02 | 13-Capillary cartridges for Size based Separation Compatible with Jess™, Abby™ and Wes™ systems ONLY. Includes 8 pack of capillaries required to perform for size assays. Sizing: 8 cartridges - 13 capillaries/cartridge. |